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- Title
Molecular characterization of a distinct begomovirus species from Vernonia cinerea and its associated DNA-β using the bacteriophage Φ29 DNA polymerase.
- Authors
Packialakshmi, R. M.; Srivastava, N.; Girish, K. R.; Usha, R.
- Abstract
Vernonia cinerea plants with yellow vein symptoms were collected around crop fields in Madurai. A portion (550 bp) of the AV1 gene amplified using degenerate primers from the total DNA purified from diseased leaf sample was cloned and sequenced. Specific primers derived from the above sequence were used to amplify 2,745 nucleotides with the typical genome organization of begomoviral DNA A (EMBL Accession No. AM182232). Sequence comparison with other begomoviruses revealed the greatest identity (82.4%) with Emilia yellow vein virus (EmYVV-[Fz1]) from China and less than 80% with all other known begomoviruses. The International Committee on Taxonomy of Viruses (ICTV) has therefore recognized Vernonia yellow vein virus (VeYVV) as a distinct begomovirus species. Conventional PCR could not amplify the DNA B or DNA β from the diseased tissue. However, the β DNA (1364 bp) associated with the disease was obtained (Accession No. FN435836) by the rolling circle amplification–restriction fragment length polymorphism method (RCA-RFLP) using Φ29 DNA polymerase. Sequence analysis shows that DNA β of VeYVV has the highest identity (56.8%) with DNA β of Sigesbeckia yellow vein Guangxi betasatellite (SibYVGxB-[CN: Gx111:05]) and 56–53% with DNA β associated with other begomoviruses. This is the first report of the molecular characterization of VeYVV from V. cinerea in India. The complete molecular characterization, phylogenetic analysis, and putative recombination events in VeYVV are reported.
- Subjects
VERNONIA; BACTERIOPHAGES; DNA polymerases; GENE amplification; PLANT genetics
- Publication
Virus Genes, 2010, Vol 41, Issue 1, p135
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-010-0484-5