We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Heterogeneity in the sensitivity of microtubules of Giardia lamblia to the herbicide oryzalin.
- Authors
Terra, Letícia L.; Campanati, Loraine; De Souza, Wanderley
- Abstract
Giardia lamblia is a protozoan that inhabits the small intestine of vertebrates, attaching to the epithelial cells by means of cytoskeletal elements. G. lamblia trophozoites possess several microtubular structures, namely the adhesive disc, the median body, the funis and the four pairs of flagella. Several drugs that target cytoskeletal proteins have been used in the study of cytoskeletal function and dynamics. In this work, we used oryzalin, which binds to α-tubulin, as a tool to study the Giardia cytoskeleton. The trophozoites were treated with oryzalin, and its effects were analysed by immunofluorescency, transmission and scanning electron microscopies. Oryzalin inhibited Giardia proliferation. Treated cells were not able to complete cell division and had flagella showing extensive shortening. Strikingly, the drug did not interfere with the adhesive disc, in contrast to what happens when other drugs are used.
- Subjects
MICROTUBULES; CYTOSKELETAL proteins; GIARDIA lamblia; IMMUNOFLUORESCENCE; SCANNING electron microscopy
- Publication
Parasitology Research, 2010, Vol 107, Issue 1, p47
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-010-1831-0