We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
SEPARATION OF MOUSE BONE MARROW CELLS USING WHEAT GERM AGGLUTININ AFFINITY CHROMATOGRAPHY.
- Authors
Nicola, N. A; Burgess, A. W.; Metcalf, D.; Battye, F. L.
- Abstract
Moose bone marrow cells were fractionate on columns of wheat germ agglutinin-Sepharose 6MB (WGA-Sepharose) and conditions established for specific binding and cell enrichment in the elute fractions. A small proportion (7%) of the cells applied did not bind to the column and 60% of these were lymphocyte-like cells. Twice as many cells were elute from the WGA-Sepharose column using N-acetyl-D-glucosamine (GlcNAc), and 80S of these cells were polymorphonuclear granulocyte's and metamyelocytes, These cells were only released from the matrix in the presence of GleNAc at high buffer flow rates (< 4 ml/min).
- Subjects
IMMUNE system; WHEAT germ; WHEAT; AGGLUTININS; AGGLUTINATION; COLD agglutinin syndrome
- Publication
Australian Journal of Experimental Biology & Medical Science, 1978, Vol 56, Issue 6, p663
- ISSN
0004-945X
- Publication type
Article
- DOI
10.1038/icb.1978.74