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- Title
366. Suicide Gene Therapy of Glioma Using Genetically Engineered Human Mesenchymal Stem Cells.
- Authors
Namba, Hiroki; Shaoyi Li; Tokuyama, Tsutomu; Yamamoto, Junkoh; Yokota, Naoki
- Abstract
We tested anti-tumor activity of genetically engineered mesenchymal stem cells (MSCs) derived from the human adult bone marrow against glioma cells. MSCs were transduced with the herpes simplex virus-thymidine kinase gene (MSCtk cells) and mixed with various glioma cell lines (two human glioma cell lines, A-172 and T98G and C6 rat glioma cell line) and examined in vitro and in vivo bystander tumoricidal effect. When cultured under the presence of ganciclovir (GCV), a complete tumor growth inhibition was observed in all cell lines. Nude mice were implanted into the brain with a mixture of MSCtk and glioma cells and administered with GCV or physiological saline (PS). In the PS group, a large tumor developed and all mice died of tumor in 3 weeks. When implanted with a mixture of MSCtk and human glioma cells and treated with GCV, no tumors were observed and mice survived more than 100 days due to a potent tumoricidal bystander effect between MSCtk and tumor cells. Even in the mice implanted with MSCtk and rat glioma cells, 2 out of 6 mice survived. In our previous rat study, we demonstrated an established glioma was successfully treated with genetically engineered neural stem cells. In the present study, we demonstrated that MSCtk cells also had a similar anti-tumor activity as the neural stem cells. Therefore, “MSCtk therapy” using MSCs that can be easily obtained from adult human bone marrow will be a feasible and promising strategy for therapy of malignamt gliomas, which are fatal at the present time.Molecular Therapy (2006) 13, S139–S139; doi: 10.1016/j.ymthe.2006.08.424
- Subjects
STEM cells; LABORATORY mice; TUMORS; GENE therapy; IMMUNE system; GENETIC engineering; HERPES simplex virus
- Publication
Molecular Therapy, 2006, Vol 13, pS139
- ISSN
1525-0016
- Publication type
Article
- DOI
10.1016/j.ymthe.2006.08.424