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- Title
Lentivirus‐mediated subcutaneous JAM‐A modification promotes skin wound healing in a mouse model by strengthening the secretory function and proliferation of fibroblasts.
- Authors
Gong, Teng; Wu, Minjuan; Fan, Xiaoming; Chen, Zhaohong; Xia, Zhaofan
- Abstract
A better understanding of the molecular regulation of wound healing may provide novel therapeutic targets. A previous study revealed that junctional adhesion molecule A (JAM‐A)‐modified mesenchymal stem cells promoted wound healing. However, whether direct JAM‐A modification in the skin wound edge area accelerates the wound repair process is not clear. We determined whether JAM‐A modification at the skin wound edge accelerated the wound healing process. We established JAM‐A modification mouse wound models and mouse primary fibroblast cell models. Wound pictures were taken to compare the wound size. H&E staining was performed to monitor the morphology of the wound and quality of the newborn skin. CCK‐8 assays and immunofluorescence (IF) for Ki67 were used to measure the cell proliferation of mouse primary fibroblasts. Quantitative real‐time PCR, immunohistochemistry, IF, and Western blot analysis were used to detect bFGF and EGF expression in vivo and in vitro. The JAM‐A‐overexpressing group exhibited a smaller residual wound size than the control group at Day 7. Thicker epidermal layers and more hair follicle‐like structures were found in the JAM‐A‐overexpressing group at Day 21. Cell proliferation capacity was higher in JAM‐A‐modified mouse fibroblasts. Elevated levels of bFGF and EGF were found in the JAM‐A‐modified group in vivo and in vitro. JAM‐A modification significantly promoted fibroblast proliferation and wound healing. Increased levels of bFGF and EGF growth factors may be part of the mechanism.
- Subjects
LABORATORY mice; EPIDERMAL growth factor; ANIMAL disease models; FIBROBLASTS; HEALING; WESTERN immunoblotting
- Publication
Cell Biology International, 2022, Vol 46, Issue 8, p1227
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.11808