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- Title
Isolation of the promoter of a root cap expressed pectinmethylesterase gene fromPisum sativumL. (rcpme1) and its use in the study of gene activity.
- Authors
Zhu, Y.; Wen, F.; Zhao, X.; Hawes, M. C.
- Abstract
A genomic clone of a pea pectinmethylesterase encoding gene,rcpme1, was isolated; the promoter region was found to include regions of homology to phenylalanine ammonia lyase (PAL) and nodulin gene promoters.Agrobacterium rhizogenesmediated hairy roots were used forrcpme1expression and functional analysis in pea. Patterns ofrcpme1expression in cultured hairy roots, measured usinguidA encodingß-glucuronidase (GUS) as a reporter gene, were distinct from patterns which occur in normal pea roots. No reporter gene expression occurred in transgenicArabidopsis thaliana, whose roots do not produce border cells. Border cell number from transgenic hairy roots expressingrcpme1anti-sense mRNA under the control of its 2.75 kb 5' flanking sequence was reduced by>50%. Nodulation genes ofRhizobium leguminosarumwere used as a marker to document that roots with reduced production of border cells and other root cap exudates have a corresponding reduction in levels of biologically active signal molecules. Direct measurements were used to confirm that most of the exudate harvested from young, unwounded roots of normal pea plants is derived from the root tip region wherercpme1is expressed. The potential application of thercpme1gene as a molecular marker for root exudate production is discussed.
- Subjects
CLONING; GENETIC engineering; PEAS; GENETIC recombination; GENE expression; CLONE cells; CYTOLOGY; HARVESTING
- Publication
Plant & Soil, 2004, Vol 265, Issue 1/2, p47
- ISSN
0032-079X
- Publication type
Article
- DOI
10.1007/s11104-005-8468-2