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- Title
Molecular Signatures of Kidney Antibody–Secreting Cells in Lupus Patients With Active Nephritis Upon Immunosuppressive Therapy.
- Authors
Crickx, Etienne; Tamirou, Farah; Huscenot, Tessa; Costedoat‐Chalumeau, Nathalie; Rabant, Marion; Karras, Alexandre; Robbins, Ailsa; Fadeev, Tatiana; Le Guern, Véronique; Remy, Philippe; Hummel, Aurélie; Aydin, Selda; Lauwerys, Bernard; Weill, Jean‐Claude; Reynaud, Claude‐Agnès; Houssiau, Frédéric; Mahévas, Matthieu
- Abstract
Objective: This study was undertaken to characterize kidney and urine antibody‐secreting cells (ASCs) from patients with active lupus nephritis, before and after induction therapy. Methods: We included patients with biopsy‐proven active lupus nephritis and performed anti‐CD138 staining of kidney biopsy samples to visualize ASCs. We performed single‐cell gene expression profiling on sorted ASCs from fresh biopsy samples using multiplex reverse transcriptase–polymerase chain reaction. We used a gene set that allowed for the study of ASC maturation from plasmablasts to long‐lived plasma cells. We quantified urine ASCs from untreated patients with lupus nephritis at diagnosis and after 6 months of prospective follow‐up during induction therapy. Results: The number of kidney CD138+ ASCs in 46 untreated patients with lupus nephritis was correlated with a low estimated glomerular filtration rate and with tubulointerstitial damage. Most kidney ASCs from 3 untreated patients had a plasmablast molecular signature; in contrast, in 4 patients with refractory lupus nephritis, the kidney ASCs were mainly long‐lived plasma cells, representing an ASC transcriptional profile similar to that in the bone marrow of 2 healthy donors. Some urine ASCs with a plasmablast signature were detected in patients with untreated active lupus nephritis. The presence of urine ASCs at 6 months was associated with treatment failure. Conclusion: Our results suggest potential for ASC‐directed therapy in refractory lupus nephritis.
- Subjects
REVERSE transcriptase polymerase chain reaction; GLOMERULAR filtration rate; KIDNEYS; LUPUS nephritis; BIOPSY; IMMUNOSUPPRESSION; TREATMENT failure; GENE expression profiling; GLYCOPROTEINS; POLYMERASE chain reaction; BONE marrow; IMMUNOGLOBULIN producing cells
- Publication
Arthritis & Rheumatology, 2021, Vol 73, Issue 8, p1461
- ISSN
2326-5191
- Publication type
Article
- DOI
10.1002/art.41703