We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Activation of adipose tissue macrophages in obese mice does not require lymphocytes.
- Authors
Behan, James W.; Ehsanipour, Ehsan A.; Sheng, Xia; Pramanik, Rocky; Wang, Xingchao; Hsieh, Yao‐Te; Kim, Yong‐Mi; Mittelman, Steven D.
- Abstract
Objective Macrophages which infiltrate adipose tissue and secrete proinflammatory cytokines may be responsible for obesity-induced insulin resistance. However, the reason why macrophages migrate into adipose tissue and become activated remains unknown though some studies suggest that this may be regulated by T and B lymphocytes. In this study, it has been tested whether T and B lymphocytes and natural killer (NK) cells were necessary for the obesity-induced activation of macrophages in adipose tissue. Design and Methods NOD/SCID/IL2-receptor gamma-chain knockout (NSG) mice, which lack mature T and B lymphocytes and NK cells, were made obese by selectively reducing litters and weaning onto a high-fat diet. Mice were then maintained on the diet for 10-11 weeks. Results Adipose tissue from obese NSG mice had more activated macrophages than nonobese mice. These macrophages were found in 'crown-like structures' surrounding adipocytes, and expressed higher levels of the inflammatory cytokine TNF-α. However, obesity did not impair glucose tolerance in the NSG mice. Conclusions These studies demonstrated that T and B lymphocytes and NK cells are not necessary for adipose tissue macrophage activation in obese mice. T and B lymphocytes and/or NK cells may be necessary for the development of obesity-induced impaired glucose tolerance.
- Subjects
ADIPOSE tissues; MACROPHAGE activation; INSULIN resistance; CYTOKINES; LYMPHOCYTES; GLUCOSE tolerance tests
- Publication
Obesity (19307381), 2013, Vol 21, Issue 7, p1380
- ISSN
1930-7381
- Publication type
Article
- DOI
10.1002/oby.20159