We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything.
- Authors
Kralik, Petr; Ricchi, Matteo
- Abstract
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique.
- Subjects
DIAGNOSTIC use of polymerase chain reaction; THERMUS aquaticus; DIAGNOSTIC microbiology; PATHOGENIC microorganisms
- Publication
Frontiers in Microbiology, 2017, Vol 8, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2017.00108