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- Title
A Comparative Study of Oleuropein Extraction from Wild Olive Leaves (Olea europea subsp. oleaster , Hoffmanns. & Link), Its Gastrointestinal Stability, and Biological Potential.
- Authors
Soldo, Barbara; Bilušić, Tea; Giacometti, Jasminka; Ljubenkov, Ivica; Čikeš Čulić, Vedrana; Bratanić, Andre; Bošković, Perica; Šola, Ivana; Ilić, Krunoslav
- Abstract
Olive leaves are the richest source of phenolic compounds, particularly oleuropein, which has many beneficial effects on human health. This study compares the effect of three extraction techniques: ultrasonic-solvent extraction, microwave-assisted extraction, and supercritical CO2 extraction of freeze-dried wild olive leaves (Olea europea subps. oleaster, Hoffmanns. & Link) on oleuropein concentration in obtained extracts. The extract with the highest concentration of oleuropein was obtained after low-frequency ultrasound solvent extraction (WOLE-S) (71.97 µg/mL). Oleuropein from that extract showed low stability after simulated gastric digestion with human gastric juices (35.66%) and high stability after 60 min of intestinal phase (65.97%). The highest antiproliferative activity of WOLE-S and pure oleuropein was detected against T24 cancer cells. The antiproliferative activity of WOLE-S against MD-MBA-123, A549, and A1235 cells was moderate, while the activity of oleuropein was high. WOLE-S significantly down-regulated the expression of IL-6 in A549 cells, indicating a possible anti-inflammatory effect in lung cancer cells. The expression of IL-8 was slightly reduced by treating the cells with WOLE-S, while the expression of IL-10 was not altered by the treatment with WOLE-S or oleuropein.
- Subjects
OLIVE leaves; EXTRACTION techniques; SOLVENT extraction; GASTRIC juice; OLIVE; CANCER cells
- Publication
Applied Sciences (2076-3417), 2024, Vol 14, Issue 2, p869
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app14020869