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- Title
Somatostatin receptor subtypes mediate contractility on human colonic smooth muscle cells.
- Authors
Corleto, V. D.; Severi, C.; Romano, G.; Tattoli, I.; Weber, H. C.; Stridsberg, M.; Rindi, G.; Campanini, N.; Tomassoni, F.; Pagotto, U.; Coy, D. H.; Jensen, R. T.; Fave, G. Delle
- Abstract
This study demonstrates the expression of functional somatostatin receptor (sstr) subtypes in human circular and longitudinal colonic smooth muscle cells (SMC). Native somatostatin (SS) and sstr subtype-specific analogues were used to characterize the sstr subtypes present in both cell types by contraction/relaxation studies. Qualitative and quantitative mRNA analysis and immunohistochemistry of sstr subtypes were also carried out. sstr subtype 2 mRNA was expressed in circular SMC, and various levels of subtypes 1, 2 and 3 mRNA were expressed in longitudinal colonic SMC. Native SS and each subtype-specific analogue exerted a modest, but significant, contraction, although inhibition of carbachol-induced contraction (relaxation) was the main effect on SMC from both layers. CH-288, a sstr subtype 1-specific analogue, and octreotide, a sstr subtype 2-specific analogue, were the most effective relaxant analogues on longitudinal and circular SMC, respectively. sstr subtypes display a distinct expression pattern on human colonic SMC; on circular SMC, subtype 2 is the only sstr, whereas sstr subtypes 1, 2 and 3 are expressed on human SMC isolated from the longitudinal layer. The contractile effects of SS are mediated through sstr subtype 2 and sstr subtype 1 on circular and longitudinal human colonic SMC, respectively.
- Subjects
SOMATOSTATIN; GASTROINTESTINAL hormones; CELL contraction; SMOOTH muscle diseases; MESSENGER RNA; IMMUNOHISTOCHEMISTRY
- Publication
Neurogastroenterology & Motility, 2006, Vol 18, Issue 3, p217
- ISSN
1350-1925
- Publication type
Article
- DOI
10.1111/j.1365-2982.2005.00752.x