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- Title
Supplementation with an insoluble fiber obtained from carob pod (Ceratonia siliqua L.) rich in polyphenols prevents dyslipidemia in rabbits through SIRT1/PGC-1α pathway.
- Authors
Valero-Muñoz, María; Ballesteros, Sandra; Ruiz-Roso, Baltasar; Pérez-Olleros, Lourdes; Martín-Fernández, Beatriz; Lahera, Vicente; de las Heras, Natalia
- Abstract
Purpose: To investigate the mechanism implicated in the effect of an insoluble fiber (obtained from carob pod) rich in polyphenols (IFCP) in lipid metabolism in the liver.Methods: Male New Zealand rabbits were fed with the following diets for 8 weeks: control diet (CT group), dyslipidemic diet supplemented with 0.5% cholesterol + 14% coconut oil (DL group) and dyslipidemic diet containing 0.5% cholesterol + 14% coconut oil plus 3% IFCP (DL + IFCP group).Results: Dyslipidemic diet with IFCP was able to reduce development of mixed dyslipidemia, liver relative weight and collagen I protein expression compared to DL rabbits. Analyses of the main enzymes implicated in cholesterol and triglycerides metabolism revealed that IFCP increased hepatic concentration of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) and cytochrome P450, family 7, subfamily a, polypeptide 1C (CYP7A1) (82.34, 114.42%, respectively) as well as protein expression of LDL receptor (42.48%) in DL rabbits. Importantly, IFCP also increased hepatic lipase (HL) levels (91.43%) and decreased glycerol phosphate acyltransferase (GPAT) and sterol regulatory element-binding protein 1C (SREBP1c) liver expression levels (20.38 and 41.20%, respectively). Finally, sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1α) hepatic expression increased in DL + IFCP group compared with DL (159.81 and 48.00%, respectively).Conclusions: These findings show that IFCP is able to abrogate the deleterious effects of hepatic dyslipidemia by modulating SIRT1 and PGC-1α pathways.
- Subjects
LIPID metabolism; COCONUT oil; ANIMAL experimentation; ANTILIPEMIC agents; CELL receptors; CELLULAR signal transduction; COLLAGEN; CHOLESTEROL content of food; DIETARY fiber; HYPERLIPIDEMIA; LIPASES; LIPIDS; LIVER; PEPTIDES; POLYPHENOLS; PROTEINS; RABBITS; TRANSFERASES; PLANT extracts; TREATMENT effectiveness; DESCRIPTIVE statistics; CYTOCHROME P-450; PHARMACODYNAMICS; THERAPEUTICS
- Publication
European Journal of Nutrition, 2019, Vol 58, Issue 1, p357
- ISSN
1436-6207
- Publication type
Article
- DOI
10.1007/s00394-017-1599-4