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- Title
The Production of Polyhydroxybutyrate by Two-Step Fermentation and the Application of Polyhydroxybutyrate as a Novel Substrate for a Biolubricant.
- Authors
Keunun, Piyatida; Rakkarn, Thanaphorn; Yunu, Tewan; Paichid, Nisa; Sangkharak, Kanokphorn; Prasertsan, Poonsuk
- Abstract
Two-step fermentation was utilized to increase PHB production. Cupriavidus necator was firstly cultivated in molasses medium for 144 h. Afterward, cells were recovered and extracted for PHB. The highest biomass (2.10 g/L) and PHB (62.38% of DCW) were obtained in the first reactor after 108 h of incubation. Filtrate after cell recovery were overflowed to the second reactor. Filtrate after cell recovery contained sugar and organic acids. Therefore, it was suitable to be re-utilized as substrate for PHB. In the second reactor, 10% of C. necator was inoculated and incubated for 120 h. The highest biomass (1.67 g/L) and PHB (67.66% of DCW) were achieved after 96 h of incubation. PHB was further utilized as substrate for biolubricant. The production of biolubricant involved a two-stage transesterification. The biolubricant from PHB passed the ISO VG-15 commercial standards for hydraulic systems and many other industrial applications.
- Subjects
POLYHYDROXYBUTYRATE; LUBRICATION &; lubricants; FERMENTATION; METHYL formate; TRANSESTERIFICATION; MOLASSES; BIOMATERIALS; BIOLOGICAL specimen analysis
- Publication
Journal of Polymers & the Environment, 2018, Vol 26, Issue 6, p2459
- ISSN
1566-2543
- Publication type
Article
- DOI
10.1007/s10924-017-1140-0