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- Title
Basolateral Junctions Utilize Warts Signaling to Control Epithelial-Mesenchymal Transition and Proliferation Crucial For Migration and Invasion of Drosophila Ovarian Epithelial Cells.
- Authors
Min Zhao; Szafranski, Przemyslaw; Hall, Chad Albert; Goode, Scott
- Abstract
Fasciclin2 (Fas2) and Discslarge (Dig) localize to the basolateral junction (BLJ) of Drosophila follicle epithelial cells and inhibit their proliferation and invasion. To identify a BLJ signaling pathway we completed a genomewide screen for mutants that enhance dig tumorigenesis. We identified two genes that encode known BLJ scaffolding proteins, lethal giant larvae (lgl) and scribble (scrib), and several not previously associated with BLJ function, including warts (wts) and roughened `e (roe), which encode a serine-threonine kinase and a transcription factor, respectively. Like scrib, wts and roe also enhance Fas2 and igltumorigenesis. Further, scrib, wts, and roeblock border cell migration, and cause noninvasive tumors that resemble digpartial loss of function, suggesting that the BLJ utilizes Wts signaling to repress EMT and proliferation, but not motility. Apicolateral junction proteins Fat (Ft), Expanded (Ex),and Merlin (Mer) either are not involved in these processes, or have highly spatio-temporally restricted roles, diminishing their significance as upstream inputs to Wts in follicle cells. This is further indicated in that Wts targets, CyclinE and DIAP1, are elevated in Fas2, dig, IgI, wts, and roe cells, hut not Fat, cx, or mercells. Thus, the RU appears to regulate epithelial polarity and dynamics not only as a localized scaffold, but also by communicating signals to the nucleus. Wts maybe regulated by distinctjunction inputs depending on developmental context.
- Subjects
EPITHELIAL cells; WARTS; GENES; PROTEINS; TUMORS; CELL migration
- Publication
Genetics, 2008, Vol 178, Issue 4, p1947
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.108.086983