We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A 2600-Locus Chromosome Bin Map of Wheat Homoeologous Group 2 Reveals Interstitial Gene-Rich Islands and Colinearity With Rice.
- Authors
Conley, E.J.; Nduati, V.; Gonzalez-Hernandez, J.L.; Mesfin, A.; Trudeau-Spanjers, M.; Chao, S.; Lazo, G.R.; Hummel, D.D.; Anderson, O.D.; Qi, L.L.; Gill, B.S.; Echalier, B.; Linkiewicz, A.M.; Dubcovsky, J.; Akhunov, E.D.; Dvořák, J.; Peng, J.H.; Lapitan, N.L.V.; Pathan, M.S.; Nguyen, H.T.
- Abstract
The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share syntenous blocks with rice chromosomes 4 and 7.
- Subjects
WHEAT genetics; PLANT genetics; CROP genetics; PLANT genomes; PLANT chromosomes; NUCLEOTIDE sequence
- Publication
Genetics, 2004, Vol 168, Issue 2, p625
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.104.034801