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- Title
CALLUS FORMATION FROM ISOLATED MICROSPORE CULTURE IN RADISH (RAPHANUS SATIVUS L.).
- Authors
Tuncer, B.
- Abstract
In this study, the effects of applications of colchicine on microspore cultures were evaluated in 4 radish (Raphanussativus L.) varieties. For this purpose, 5 different concentrations of colchicine (0, 10, 25, 50, and 75 mg L-1) were applied under dark conditions at 4°C to microspores that had been isolated in Gamborg's medium containing 13% (w/v)sucrose (B5-13). After the application of colchicine the microspores were kept for 1 day at 32.5°C in1/2 x Nitsch and Nitschliquid media supplemented with13% (w/v)sucrose (1/2 NLN-13) at a density of 40.000 microspore mL-1and transferred for culturing under dark conditions at 25°C. Colonies of callus were observed in Petri dishes 4-5 weeks after the microspore isolation. In this study, the 50 mg L-1 dose of colchicine was found to be more effective in terms of callus formation. The highest callus formation was achieved from the large round radish (cv. Burkır) (6.63 calli/Petri dish)and the standard small rooted radish (cv. Cherry Belle)(5.73 calli/Petri dish) varieties.
- Subjects
CALLUS; RADISHES; MICROSPORES (Botany); COLCHICINE; NONSTEROIDAL anti-inflammatory agents
- Publication
JAPS: Journal of Animal & Plant Sciences, 2017, Vol 27, Issue 1, p277
- ISSN
1018-7081
- Publication type
Article