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- Title
RESISTENCIA ANTIBIÓTICA EN CEPAS AISLADAS DE CARNES DE UN MERCADO MUNICIPAL DE LIMA CERCADO - PERÚ.
- Authors
CRISTÓBAL DELGADO, Ruth; DIOSES FLORES, Bertha; AMPUERO LEÓN, Angela; MAURTUA TORRES, Dora
- Abstract
Background: The antibiotic resistance has become a problem for consumers and meat and poultry industries. The antibiotics, including cephalosporins from third and fourth generation, prescribed for humans are also used for animals, mainly as growth promoters. This indiscriminate use is increasing the number of resistant strains like E. coli beta-lactamase producers, becoming a great risk in case of strain transmission. In Peru, where most of the population is meat-consumer, there is not much information about this topic. Objectives: Determine the phenotypic antibiotic resistance from isolated strains of meat expended in a municipal market of Lima. Methods: The meat samples were from beef, pork and chicken. For the isolation of Enterobacteriaceae and S. aureus, decimal serial dilutions were performed and then were streaked on MacConkey and Baird Parker medium by dissemination. For Salmonella detection, the sample was placed in lactose broth and later, inoculated in Selenite and Tetrationate broths. For Campylobacter detection, another portion of sample was placed in Nutritive Broth with 0.6% of yeast extract and streaked in Agar Columbia with sheep blood. The colonies were selected from every streaked medium. Identification was conducted according to Bergey's Manual. For determination of antibiotic resistance, the Kirby-Bauer method was used according to CLSI's Manual 2016. Results: Most isolated strains were S. aureus in pork meat, coliforms like Citrobacter spp. and K. pneumoniae in beef meat, and E. coli and Proteus spp. in chicken meat. The determination of antibiotic resistance showed that for the 9 S. aureus isolates, 4 are beta-lactamase producers and for the 15 E. coli isolates, 4 are producers of BLEE and only 1 of carbapenemases. Conclusions: The E. coli and S. aureus isolates showed a phenotypic resistance to antibiotics beta-lactams, which represents a risk of transfer to the human for meat consumption.
- Publication
Vitae (01214004), 2016, Vol 23, pS85
- ISSN
0121-4004
- Publication type
Article