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- Title
Relationship between anti-Müllerian hormone and antral follicle count across the menstrual cycle using the Beckman Coulter Access assay in comparison with Gen II manual assay.
- Authors
Schiffner, Julia; Roos, Judith; Broomhead, David; van Helden, Joseph; Godehardt, Erhard; Fehr, Daniel; Freundl, Günter; Johnson, Sarah; Gnoth, Christian
- Abstract
Background: The study aim was to validate Beckman Coulter's fully automated Access Immunoassay System (BC Access assay) for anti-Müllerian hormone (AMH) and compare it with Beckman Coulter's Modified Manual Generation II assay (BC Mod Gen II), with regard to cycle AMH fluctuations and antral follicle counts. Methods: During one complete menstrual cycle, transvaginal ultrasound was performed on regularly menstruating women (n = 39; 18-40 years) every 2 days until the dominant ovarian follicle reached 16 mm, then daily until observed ovulation; blood samples were collected throughout the cycle. Number and size of antral follicles was determined and AMH levels measured using both assays. Results: AMH levels measured by the BC Access assay vary over ovulatory menstrual cycles, with a statistically significant pre-ovulatory decrease from - 5 to + 2 days around objective ovulation. Mean luteal AMH levels were significantly lower (- 7.99%) than mean follicular levels but increased again towards the end of the luteal phase. Antral follicle count can be estimated from AMH (ng/mL, BC Access assay) concentrations on any follicular phase day. BC Access assay-obtained AMH values are considerably lower compared with the BC Mod Gen II assay (- 19% on average); conversion equation: AMH BC Access (ng/mL) = 0.85 [AMH BC Mod Gen II (ng/mL)]0.95. Conclusions: AMH levels vary throughout the cycle, independently of assay utilised. A formula can be used to convert BC Access assay-obtained AMH levels to BC Mod Gen II values. The number of antral follicles can be consistently estimated from pre-ovulatory AMH levels using either assay.
- Subjects
ANTI-Mullerian hormone; MENSTRUAL cycle; SEXUAL cycle; ENDOCRINE gynecology; PHYSIOLOGY of women; IMMUNOASSAY; PROTEIN hormones; MULLERIAN ducts
- Publication
Clinical Chemistry & Laboratory Medicine, 2017, Vol 55, Issue 7, p1025
- ISSN
1434-6621
- Publication type
Article
- DOI
10.1515/cclm-2016-0609