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- Title
Chemometric analysis of attenuated total reflectance infrared spectra of Proteus mirabilis strains with defined structures of LPS.
- Authors
Zarnowiec, Paulina; Mizera, Andrzej; Chrapek, Magdalena; Urbaniak, Mariusz; Kaca, Wieslaw
- Abstract
Proteus spp. strains are some of the most important pathogens associated with complicated urinary tract infections and bacteremia affecting patients with immunodeficiency and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods have been developed for this pathogen. However, these methods are labor intensive and time consuming. We evaluated a new method of differentiation between strains. A collection of Proteus spp. strains was analyzed by attenuated total reflectance Fourier transform infrared (ATR FT-IR) spectroscopy in the mid-infrared region. ATR FT-IR spectroscopy used in conjunction with a diamond ATR accessory directly produced the biochemical profile of the surface chemistry of bacteria. We conclude that a combination of ATR FT-IR spectroscopy and mathematical modeling provides a fast and reliable alternative for discrimination between Proteus isolates, contributing to epidemiological research.
- Subjects
CHEMOMETRICS; PROTEUS (Bacteria); LIPOPOLYSACCHARIDES; URINARY tract infections; URINARY catheterization; FOURIER transform infrared spectroscopy; EPIDEMIOLOGICAL research
- Publication
Innate Immunity, 2016, Vol 22, Issue 5, p325
- ISSN
1753-4259
- Publication type
Article
- DOI
10.1177/1753425916647470