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- Title
High-throughput mapping of the chromatin structure of human promoters.
- Authors
Ozsolak, Fatih; Song, Jun S.; Liu, X. Shirley; Fisher, David E.
- Abstract
Our understanding of how chromatin structure influences cellular processes such as transcription and replication has been limited by a lack of nucleosome-positioning data in human cells. We describe a high-resolution microarray approach combined with an analysis algorithm to examine nucleosome positioning in 3,692 promoters within seven human cell lines. Unlike unexpressed genes without transcription-preinitiation complexes at their promoters, expressed genes or genes containing preinitiation complexes exhibit characteristic nucleosome-free regions at their transcription start sites. The combination of these nucleosome data with chromatin immunoprecipitation–chip analyses reveals that the melanocyte master regulator microphthalmia-associated transcription factor (MITF) predominantly binds nucleosome-free regions, supporting the model that nucleosomes limit sequence accessibility. This study presents a global view of human nucleosome positioning and provides a high-throughput tool for analyzing chromatin structure in development and disease.
- Subjects
TRANSCRIPTION factors; GENE mapping; CHROMOSOME abnormalities; IMMOBILIZED nucleic acids; MELANOCYTES; MICROPHTHALMUS
- Publication
Nature Biotechnology, 2007, Vol 25, Issue 2, p244
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt1279