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- Title
Rapid Elevation of Calcium Concentration in Cultured Dorsal Spinal Cord Astrocytes by Corticosterone.
- Authors
Zeng, Junwei; Li, Min; Xiao, Zhi; Chen, Yuanshou; Chang, Quanzhong; Tian, Hong; Jin, Huan; Liu, Xiaohong
- Abstract
In addition to the classic genomic effects, increasing evidence suggests that GC can generate multiple rapid effects on many tissues and cells through nongenomic pathway. In the present study, the effects of corticosterone (CORT) on the intracellular calcium concentration ([Ca]i) in cultured dorsal spinal cord astrocytes were detected with confocal laser scanning microscopy using fluo-4/AM as a calcium fluorescent indicator that could monitor real-time alterations of [Ca]i. CORT (0.01-10 μM) caused a rapid increase in [Ca]i with a dose-dependent manner in cultured dorsal spinal cord astrocytes. The action of CORT on astrocytic [Ca]i was blocked by pertussis toxin (a blocker of G protein activation, 100 ng/ml), but was unaffected by RU38486 (glucocorticoid receptor antagonist, 10 μM). In addition, cycloheximide (protein-synthesis inhibitor, 10 μg/ml) pretreatment could not impair the CORT-evoked [Ca]i elevation. Furthermore, Ca mobilization induced by CORT was abolished by chelerythrine chloride (protein kinase C inhibitor, 10 μM), but was not impaired by H89 (protein kinase A inhibitor, 10 μM). These observations suggest that a nongenomic pathways might be involved in the effect of CORT on [Ca]i in cultured dorsal spinal cord astrocytes. In addition, our results also raise a possibility that a putative pertussis toxin-sensitive mGCR (G-protein-coupled membrane-bound glucocorticoid receptor) and the downstream activation of protein kinase C may be responsible for CORT-induced Ca mobilization in cultured dorsal spinal cord astrocytes.
- Subjects
SPINAL cord; ASTROCYTES; CORTICOSTERONE; CELL culture; INTRACELLULAR calcium; G protein coupled receptors; PROTEIN synthesis
- Publication
Neurochemical Research, 2013, Vol 38, Issue 2, p382
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-012-0929-4