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- Title
The chromosomal integration of a β-lactamase gene derived from the P-type R-factor RP1 in Escherichia coli.
- Authors
Richmond, M. H.; Sykes, R. B.
- Abstract
The R-factor RP1, which carried the markers amp neo tet, confers resistance to penicillins by specifying the synthesis of Type IIIa β-lactamase. It was detected initially in a strain of Pseudomonas aeruginosa (Sykes & Richmond, 1970) and fragments spontaneously in Ps. aeruginosa strain Ps 18 to give clones which carry only the amp marker. Such clones are said to carry the element RP 1–1 since they are still capable of specifying the transfer of ampicillin and carbenicillin resistance to appropriate recipients in mating experiments. However, unlike lines carrying RP 1, they contain no detectable extrachromosomal DNA (Ingram et al. 1972), and the likely supposition is that the amp gene in these clones has become integrated into the bacterial chromosome together with the regions necessary to allow such strains to act as amp gene donors in mating experiments. It is probably cells of Ps. aeruginosa in which the amp gene has become integrated together with an RTF region that are responsible for the transfer of chromosomal markers reported by Holloway & Stanisich (1971).
- Publication
Genetics Research, 1972, Vol 20, Issue 2, p231
- ISSN
0016-6723
- Publication type
Article
- DOI
10.1017/S0016672300013732