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- Title
The role of heparan sulfate on adhesion of 47 and 51 kDa outer membrane proteins of Helicobacter pylori to gastric cancer cells.
- Authors
López-Bolaños, Claudia C.; Guzm� E1;n-Murillo, Maria A.; Ruiz-Bustos, Eduardo; Ascencio, Felipe
- Abstract
Helicobacter pylori is a common gastrointestinal pathogenic bacterium in humans and the usual preference for the stomach’s outer membrane proteins (OMPs) are antigens involved in the adhesion process. Through SDS-PAGE and blotting analyses, using horseradish peroxidase-labeled heparan sulfate (HRP-HS) as a probe, we identified H. pylori OMPs with affinity for heparan sulfate (OMP-HS). Biotin-streptavidin bacterial-adhesion assay was used to evaluate participation of OMP-HS in the adhesion of H. pylori to semi-confluent HeLa S3 and Kato III cell monolayers. The results provide evidence that induction of antibodies against 2 OMP-HSs (HSBP-47 and HSBP-51) could reduce binding of H. pylori to both cell lines and induce detachment of cell-bound bacteria from infected cultured cells.
- Subjects
ADHESION; MEMBRANE proteins; HELICOBACTER pylori infections; GASTRIC diseases; PATHOGENIC bacteria; ANTIGENS; HORSERADISH; STREPTAVIDIN; CELL culture
- Publication
Canadian Journal of Microbiology, 2009, Vol 55, Issue 4, p450
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W08-149