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- Title
Acetylation and nitrosation of ciprofloxacin by environmental strains of mycobacteria.
- Authors
Adjei, Michael D.; Heinze, Thomas M.; Deck, Joanna; Freeman, James P.; Williams, Anna J.; Sutherland, John B.
- Abstract
To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg·L–1 ciprofloxacin. After the cultures had been centrifuged and the supernatants extracted with ethyl acetate, two metabolites were purified by using high-performance liquid chromatography. They were identified with liquid chromatography/electrospray ionization mass spectrometry and proton nuclear magnetic resonance spectroscopy. Ciprofloxacin was transformed to both N-acetylciprofloxacin (2.5%–5.5% of the total peak area at 280 nm) and N-nitrosociprofloxacin (6.0%–8.0% of the peak area) by Mycobacterium gilvum PYR-GCK and Mycobacterium sp. PYR100 but it was transformed only to N-acetylciprofloxacin by Mycobacterium frederiksbergense FAn9, M. gilvum ATCC 43909, M. gilvum BB1, Mycobacterium smegmatis mc2155, Mycobacterium sp. 7E1B1W, and Mycobacterium sp. RJGII-135. The results suggest that biotransformation may serve as a ciprofloxacin resistance mechanism for these bacteria.
- Subjects
CIPROFLOXACIN; MYCOBACTERIUM; SORBITOL; ETHYL acetoacetate; FUNGUS-bacterium relationships; ACTINOMYCETALES
- Publication
Canadian Journal of Microbiology, 2007, Vol 53, Issue 1, p144
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W06-101