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- Title
Antioxidant Activity of Guanosine Monophosphate Disodium in vitro.
- Authors
QIAN Jia; SHI Yong-hui; MA Li-qin; LE Guo-wei
- Abstract
The antioxidant activities of GMPNa2 on hydroxyl free radical ( · OH)and DPPH free radical(DPPH ·) were studied by chemical colorimetry. The splenic cells of mice were treated with H2O2 to cause oxidative stress injury and they were determined by MTT method. To investigate in vitro antioxidant effect of GMPNa2 on repairing of injury, total antioxidation capability (T-AOC), catalase (CAT) activities, glutathione peroxidase (GSH-Px) activities and diaminodiphenyl methane(MDA) in incubation medium were detected. The results indicated that GMPNa2 could scavenge free radicals in concentration dependent manner in vitro and significantly repair the injury of H2O2 in splenic cells. GMPNa2 at a concentration of 10 mmol/L showed 96. 644% inhibition ratio to hydroxyl free radical, and only 6.589% to DPPH. The hydroxyl free radical scavenging capacity of GMPNa2 was nearly to that of Vc. Compared with the injured group, the number of live cells increased significantly in H2O2 injured splenic cells, with the supplementation of 0.5,1,5 and 10 mmol/ L GMPNa2 (P < 0.05). CAT activities, GSH-Px activities and T-AOC in incubation medium were increased significantly with GMPNa2 concentration increased(P < 0.01). MDA content decreased significantly (P < 0.01) with supplementation of more than 1 mmol/L GMPNa2 to the injured group. It was concluded that GMPNa2 has the significant anti-oxidation activity.
- Subjects
ANTIOXIDANTS; CYCLIC guanylic acid; SODIUM compounds; FREE radicals; OXIDATIVE stress; GLUTATHIONE; DIAMINODIPHENYLMETHANE; LABORATORY mice
- Publication
Natural Product Research & Development, 2009, Vol 21, Issue 4, p566
- ISSN
1001-6880
- Publication type
Article