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- Title
Anti-inflammatory Activity of 3,6,3'-Trihydroxyflavone in Mouse Macrophages, In vitro.
- Authors
Eunjung Lee; Ki-Woong Jeong; Areum Shin; Yangmee Kim
- Abstract
Numerous studies have examined the role of flavonoids in modulating inflammatory responses in vitro. In this study, we found a novel flavonoid, 3,6,3'-trihydroxyflavone (1), with anti-inflammatory effects. Antiinflammatory activity and mechanism of action were examined in mouse macrophages stimulated with lipopolysaccharide (LPS). Our results showed that the anti-inflammatory effects of 1 are mediated via p38 mitogen-activated protein kinase (p38 MAPK), Jun-N terminal kinase (JNK), and the extracellularsignal- regulated kinase (ERK) pathway in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Binding studies revealed that 1 had a high binding affinity to JNK1 (1.568 x 108 M-1) and that the 3- and 6-hydroxyl groups of the C-ring and A-ring of 1 participated in hydrogen bonding interactions with the side chains of Asn114 and Lys55, respectively. The oxygen at the 3' position of the B-ring formed a hydrogen bond with side chain of Met111. Therefore, 1 could be a potential inhibitor of JNKs, with potent anti-inflammatory activity.
- Subjects
ANTI-inflammatory agents; FLAVONOIDS; LIPOPOLYSACCHARIDES; MITOGEN-activated protein kinases; EXTRACELLULAR signal-regulated kinases
- Publication
Bulletin of the Korean Chemical Society, 2014, Vol 35, Issue 11, p3169
- ISSN
0253-2964
- Publication type
Article
- DOI
10.5012/bkcs.2014.35.11.3169