We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bacterial community structure and abundances of antibiotic resistance genes in heavy metals contaminated agricultural soil.
- Authors
Zhang, Fengli; Zhao, Xiaoxue; Li, Qingbo; Liu, Jia; Ding, Jizhe; Wu, Huiying; Zhao, Zongsheng; Ba, Yue; Cheng, Xuemin; Cui, Liuxin; Li, Hongping; Zhu, Jingyuan
- Abstract
Soil contamination with heavy metals is a worldwide problem especially in China. The interrelation of soil bacterial community structure, antibiotic resistance genes, and heavy metal contamination in soil is still unclear. Here, seven agricultural areas (G1-G7) with heavy metal contamination were sampled with different distances (741 to 2556 m) to the factory. Denaturing gradient gel electrophoresis (DGGE) and Shannon index were used to analyze bacterial community diversity. Real-time fluorescence quantitative PCR was used to detect the relative abundance of ARGs <italic>sul1</italic>, <italic>sul2</italic>, <italic>tetA</italic>, <italic>tetM</italic>, <italic>tetW</italic>, one mobile genetic elements (MGE) <italic>inti1</italic>. Results showed that all samples were polluted by Cadmium (Cd), and some of them were polluted by lead (Pb), mercury (Hg), arsenic (As), copper (Cu), and zinc (Zn). DGGE showed that the most abundant bacterial species were found in G7 with the lightest heavy metal contamination. The results of the principal component analysis and clustering analysis both showed that G7 could not be classified with other samples. The relative abundance of <italic>sul1</italic> was correlated with Cu, Zn concentration. Gene <italic>sul2</italic> are positively related with total phosphorus, and <italic>tetM</italic> was associated with organic matter. Total gene abundances and relative abundance of <italic>inti1</italic> both correlated with organic matter. Redundancy analysis showed that Zn and <italic>sul2</italic> were significantly related with bacterial community structure. Together, our results indicate a complex linkage between soil heavy metal concentration, bacterial community composition, and some global disseminated ARG abundance.
- Subjects
BACTERIAL communities; ANTIBIOTIC residues; GENE expression; HEAVY metals; RADIOACTIVE substances
- Publication
Environmental Science & Pollution Research, 2018, Vol 25, Issue 10, p9547
- ISSN
0944-1344
- Publication type
Article
- DOI
10.1007/s11356-018-1251-8