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- Title
Important Role of Catalase in the Production of β-carotene by Recombinant Saccharomyces cerevisiae under HO Stress.
- Authors
Guo-liang Yan; Heng-yu Liang; Zhi-qun Wang; Xiao-fan Yang; Dan Liu; Jin-fu Liu; Chang-qing Duan
- Abstract
The effect of HO supplement on cell growth and β-carotene productions in recombinant Saccharomyces cerevisiae CFW-01 and CFW-01 ctt1 deficiency in cytosolic catalase were investigated in shaking flasks. The results showed that supplement of HO (0.5 and 1.0 mM) can significantly stimulate the β-carotene production. However, β-carotene levels of CFW-01 ctt1Δ under 0.5 and 1 mM HO were 16.7 and 36.7% lower than those of CFW-01, respectively. Although lacking cytosolic catalase, no significant differences in cell growth were observed between CFW-01 ctt1Δ and CFW-01 under the same level of HO stress. These results suggest that β-carotene can act as an antioxidant to protect the recombinant yeast from HO oxidative damage in the absence of cytosolic catalase. However, catalase still plays an important role in the production of β-carotene under HO stress. If catalase can not timely decompose HO, the free radicals such as OH· derived from HO can result in decrease of β-carotene concentration. Therefore, in the production of β-carotene by HO stress, not only the level of oxidative stress, but also the activities of catalase in cells should be considered.
- Subjects
CATALASE; SACCHAROMYCES cerevisiae; LEAVENING agents; FREE radical reactions; HEMOPROTEINS
- Publication
Current Microbiology, 2011, Vol 62, Issue 3, p1056
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-010-9826-8