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- Title
Micro- CT observation of angiogenesis in bone regeneration.
- Authors
Udagawa, Asami; Sato, Shuichi; Hasuike, Akira; Kishida, Mamoru; Arai, Yoshinori; Ito, Koichi
- Abstract
Objectives Restoration of an adequate blood supply is essential for the bone healing process and is key to the success of bone augmentation procedures. In this study, we evaluated angiogenesis in rat calvarial flat bone defects using in vivo microfocus computed tomography (micro- CT). Materials and methods Twenty rats were used. The calvarium was exposed and calvarial bone defects of critical (5-mm diameter) and non-critical (2.7-mm diameter) sizes were prepared. Bone regeneration and angiogenesis were evaluated by image analysis using micro- CT and histological examination. Results Critical- and non-critical-sized calvarial bone defects showed bone regeneration and angiogenesis around the midsagittal suture. Critical-sized calvarial bone defects showed approximately 1.2% reossification of the original surgical defect, whereas the non-critical-sized defects showed approximately 43.3% reossification at day 28. Furthermore, angiogenesis was observed later in the critical-sized calvarial bone defects (about 38.2%), whereas angiogenesis was observed early in the non-critical-sized calvarial bone defects (about 75.5%) at day 28. New blood vessel networks were observed around defects of both sizes. Conclusions Angiogenesis preceded bone regeneration around critical- and non-critical-sized calvarial bone defects. Angiogenesis led to full bone formation in non-critical-sized defects.
- Subjects
BONE injuries; COMPUTED tomography; BONE regeneration; BONE blood-vessels; WOUND healing; LABORATORY rats; CALVARIA
- Publication
Clinical Oral Implants Research, 2013, Vol 24, Issue 7, p787
- ISSN
0905-7161
- Publication type
Article
- DOI
10.1111/j.1600-0501.2012.02458.x