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- Title
Contribution of the Rv2333c efflux pump (the Stp protein) from Mycobacterium tuberculosis to intrinsic antibiotic resistance in Mycobacterium bovis BCG.
- Authors
Santiago Ramón-García; Carlos Martín; Edda De Rossi; José A. Aínsa
- Abstract
<sec><st>Objectives</st> To characterize the efflux pump encoded by the gene <it>Rv2333c</it> from <it>Mycobacterium tuberculosis</it>, and assess its contribution to intrinsic antibiotic resistance using <it>Mycobacterium bovis</it> BCG as a model organism. </sec> <sec><st>Methods</st> Firstly, the <it>Rv2333c</it> gene was expressed from a multicopy plasmid in <it>M. bovis</it> BCG. Secondly, the gene was inactivated in the chromosome of <it>M. bovis</it> BCG. Antibiotic susceptibility tests and tetracycline uptake/efflux experiments were carried out with the strains mentioned above. </sec> <sec><st>Results</st> When the <it>Rv2333c</it> gene was inactivated in the <it>M. bovis</it> BCG chromosome, there was a decrease in the MIC values of spectinomycin and tetracycline, and an increase in [3H]tetracycline accumulation. When the <it>Rv2333c</it> gene was cloned into a multicopy plasmid, there was an increase in the MIC values of spectinomycin and tetracycline, and a decrease in [3H]tetracycline accumulation. These results indicate that both antibiotics are substrates of the Rv2333c efflux pump, which has been named Stp, for Spectinomycin Tetracycline efflux Pump. </sec> <sec><st>Conclusions</st> The Rv2333c efflux pump (Stp protein) of <it>M. tuberculosis</it> contributes to intrinsic spectinomycin and tetracycline resistance. </sec>
- Subjects
MYCOBACTERIUM; TETRACYCLINE; MYCOBACTERIA; LUNG diseases
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2007, Vol 59, Issue 3, p544
- ISSN
0305-7453
- Publication type
Article