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- Title
单核细胞增生李斯特菌 LMXJ15 全基因组测序及分析.
- Authors
杜冬冬; 钱晶; 李思琪; 刘雯菲; 魏向利; 刘长勇; 罗瑞峰; 康立超
- Abstract
In order to understand the genomic characteristic and evolutionary relationship of Listeria monocytogenes LMXJ15 from Xinjiang, PacBio and Illumina HiSeq platforms were used to have the whole genome sequencing of LMXJ15, then the relevant software were applied to predict and annotate the sequenced data. Multilocus sequence typing (MLST) and collinearity analysis were conducted to analyze the homologies among the LM strains. The results showed that the length of LMXJ15 genome was 3 017 732 bp with a GC value of 37.81%. It was predicted to contain 3154 protein-coding genes with a total length of 2 734 315bp. The genome of LMXJ15 contained 6 rRNA operons and 57 tRNA, 88 virulence genes being predicted from the genome. Through the CRT prediction, a CRISPR sequence was found in the LMXJ15 genome sequence. A total 2 241 genes of 3 GO contents were obtained by comparing GO database. Total 88 possible virulence genes were predicted. MLST analysis showed that LMXJ15 was sequence type (ST) 8 and belonged to CC8 (clonal complex 8). Evolutionary analysis showed that the LMXJ15 had a close evolutionary relationship with two clinical IZSAM_Lm_15_17439_A144 isolates from Italy, LmN1546 isolates from Switzerland, R479a isolates from smoked salmon, and had the same ST type and serotype (1/2a), and comparative genomics among 4 LM strains showed scant collinearity. In summary, we preliminarily analyzed the genomic characteristics and virulence genes situation of LMXJ15 isolate, which may provide basic data for analysis of the evolution relationship and the virulence of L. monocytogenes in Xinjiang province.
- Subjects
XINJIANG Uygur Zizhiqu (China); ITALY; SWITZERLAND; WHOLE genome sequencing; LISTERIA monocytogenes; COMPARATIVE genomics; DATABASES; GENOMES; OPERONS; GENETIC software
- Publication
Biotechnology Bulletin, 2023, Vol 39, Issue 7, p298
- ISSN
1002-5464
- Publication type
Article
- DOI
10.13560/j.cnki.biotech.bull.1985.2022-1093