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- Title
Complete genome sequence of a phage hyperparasite of <italic>Candidatus</italic> Xenohaliotis californiensis (Rickettsiales) - a pathogen of <italic>Haliotis</italic> spp (Gasteropoda).
- Authors
Cruz-Flores, Roberto; Cáceres-Martínez, Jorge; Del Río-Portilla, Miguel Ángel; Licea-Navarro, Alexei F.; Gonzales-Sánchez, Ricardo; Guerrero, Abraham
- Abstract
Bacteriophages are recognized as major mortality agents of microbes, among them intracellular marine rickettsiales-like bacteria. Recently, a phage hyperparasite of <italic>Candidatus</italic> Xenohaliotis californiensis (<italic>C</italic>Xc) has been described. This bacterium is considered the causal agent of Withering Syndrome (WS) which is a chronic and potentially lethal disease of abalone species from California, USA and the peninsula of Baja California, Mexico. This hyperparasite which infects <italic>C</italic>Xc could be used as a biocontrol agent for WS. Therefore, it is necessary to obtain genomic information to characterize this phage. In this study, the first complete genome sequence of a novel phage, Xenohaliotis phage (p<italic>C</italic>Xc) was determined. The complete genome of p<italic>C</italic>Xc from red abalone (<italic>Haliotis rufescens</italic>) is 35,728 bp, while the complete genome of p<italic>C</italic>Xc from yellow abalone (<italic>Haliotis corrugata</italic>) is 35,736 bp. Both phage genomes consist of double-stranded DNA with a G + C content of 38.9%. In both genomes 33 open reading frames (ORFs) were predicted. Only 10 ORFs encode proteins that have identifiable functional homologues. These 10 ORFs were classified by function, including structural, DNA replication, DNA packaging, nucleotide transport and metabolism, life cycle regulation, recombination and repair, and additional functions. A PCR method for the specific detection of p<italic>C</italic>Xc was developed. This information will help to understand a new group of phages that infect intracellular marine rickettsiales-like bacteria in mollusks.
- Subjects
ABALONES; CANDIDATUS diseases; BACTERIOPHAGES; RICKETTSIALES; NUCLEOTIDE sequencing; DISEASES
- Publication
Archives of Virology, 2018, Vol 163, Issue 4, p1101
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-018-3703-3