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- Title
Biologic effects induced by peptides marked with <sup>68</sup>Ga upon human and murine malignant cells.
- Authors
Marieta, Elena Panait; Negoiță, Valentina; Bușcă, Antonela; Dumitru, Mirela; Gruia, Maria Iuliana; Chilug, Livia; Niculae, Dana; Manda, Gina
- Abstract
The authors investigated possible biological effects induced by different peptidic compounds marked with 68Ga, proposed for being used in PET imaging upon human and murine malignant tumour cells. Materials and method. In this regard, 4 tumour cell lines used frequently in the in vitro experiments have been selected: glioblastoma (U87MG), colon carcinoma (HT-29), malignant melanoma (B16F10) and pancreatic carcinoma (AR42J). At the same time, peptides with known specificity to the cell receptors of the malignant lines mentioned above have been selected, namely: peptide of type Arg-Gly-Asp (RGD) (alpha (v) beta(3) integrin receptors, synthesis analogues of somatostatin: octreotide and octreotate (SST2) and Exendin-4 glycopeptide (GLP-1). The peptides marked with 68Ga as positron emitter through the bifunctional chelating agents represented by DOTA, NOTA or NODAGA, were put into contact with the line cells which overexpress the peptidic receptors. Apoptosis (using the set Abcam Annexin V-FITC/PI apoptosis detection kit), the proliferation index and the phases of the cellular cycle (BD CycleTestTM plus DNA kit) were measured through flow cytometry (FACSCalibur, BD Biosciences, USA). Outcomes. Peptides marked with 68Ga induced apoptosis and block the cells in the phase G2/M, for the lines U87MG and AR42J. Conclusions. The results suggest the usefulness of the imaging radiopeptidic agents for the early estimate of the response to the anti-cancer therapy. These results are also promising for continuing the in vitro and in vivo evaluation of radiopeptides, for the purpose of contributing to a better understanding of the radiobiologic mechanisms by showing cell changes associated with the medical imaging procedures.
- Subjects
GALLIUM; CANCER cells; PEPTIDES
- Publication
Oncolog-Hematolog, 2017, Issue 39, p53
- ISSN
2066-8716
- Publication type
Abstract