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- Title
Comparison of the effectiveness of three different growth media for primary isolation of <em>Renibacterium salmoninarum</em> from Atlantic salmon, <em>Salmo salar</em> L., broodfish.
- Authors
Gudmundsdóttir, S.; Helgason, S.; Benediktsdóttir, E.
- Abstract
The effectiveness of three different growth media KDM-2, S-KDM and S-KDM-C for primary isolation of Renibacterium salmoninarum was examined over a 14-week incubation period. Kidney samples were taken from Atlantic salmon, Salmo salar L., broodfish returning to a sea ranch after 2 years at sea. Homogenized samples were inoculated onto two selective media, S-KDM supplemented with lamb serum and S-KDM-C supplemented with activated charcoal. The third medium was the non-selective serum supplemented KDM-2. Renibacterium salmoninarum was isolated from 112 samples on one or more of the media used. Of all positive samples, 91% were positive on S-KDM, 60% on S-KDM-C and 35% on KDM-2. These results demonstrate that selectivity significantly enhances the isolation capacity of the medium and that supplementing with serum is significantly more effective than supplementing with charcoal.
- Subjects
RENIBACTERIUM salmoninarum; ATLANTIC salmon; SERUM; CHARCOAL; KIDNEYS; FISHES
- Publication
Journal of Fish Diseases, 1991, Vol 14, Issue 1, p89
- ISSN
0140-7775
- Publication type
Article
- DOI
10.1111/j.1365-2761.1991.tb00579.x