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- Title
Bioactive L acidissima protein hydrolysates using Box-Behnken design.
- Authors
Sonawane, Sachin; Arya, Shalini
- Abstract
This study examines the extraction and hydrolysis of proteins using single factor and Box-Behnken Design (BBD). From single factor tests, optimised extraction parameters were 1% alkali concentration, 40 °C temperature, 60 min time, and 1:20 solid to alkali ratio. Under these conditions; 924.31 mg/g of total protein was obtained from Limonia acidissima (L acidissima). The maximum degree of hydrolysis was 39.82% at pH 2, enzyme to substrate ratio 2.5% (w/w), and hydrolysis time was 42.41 min using BBD design. L acidissima seed protein hydrolysate showed 32.94% DPPH and 88.18% of ABTS activity at concentration of 100 µg/ml and 1 mg/ml, respectively. Reducing power of 0.16 and metal chelating activity of 87.39% was obtained from 5 mg/ml protein hydrolysates. This implied that L acidissima seed protein hydrolysate could be utilised in protein rich product or as protein supplements.
- Subjects
LOXOSTEGE; PROTEIN hydrolysates; ANTIOXIDANTS; HYDROLYSIS; ALKALIES
- Publication
3 Biotech, 2017, Vol 7, Issue 3, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-017-0862-y