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- Title
The effects of cure of Helicobacter pylori infection on the signal transduction of gastric epithelial cells.
- Authors
Azuma, T.; Yamazaki, S.; Yamakawa, A.; Ito, Y.; Ohtani, M.; Dojo, M.; Yamazaki, Y.; Higashi, H.; Hatakeyama, M.
- Abstract
Summary Background : The CagA protein of Helicobacter pylori is directly injected from the bacteria into cells via the bacterial type IV secretion system and undergoes tyrosine phosphorylation in the gastric epithelial cells. Translocated CagA forms a physical complex with the SRC homology 2 domain (SH2)-containing tyrosine phosphatase SHP-2, which plays an important role in mitogenic signal transduction in the host cells. Aim : We examined the effect of eradication therapy on the signal transduction pathway of gastric epithelial cells induced by the CagA protein of H. pylori . Methods : Gastric biopsy samples were obtained from 20 H. pylori -positive atrophic gastritis patients before, and 3 months after, H. pylori infection eradication therapy, and subjected to immunoblot analysis to detect tyrosine phosphorylated CagA protein and CagA co-immunoprecipitated endogenous SHP-2. Results : Tyrosine phosphorylated CagA protein and CagA co-immunoprecipitated endogenous SHP-2 were detected in the gastric mucosa from H. pylori -positive atrophic gastritis patients. All H. pylori strains from these patients were cagA -positive type I strains. After curing H. pylori infection, the tyrosine phosphorylated CagA protein and CagA co-immunoprecipitated endogenous SHP-2 disappeared from the gastric mucosa. Conclusion : The cure of infection reduces the stimulated signal transduction of gastric epithelial cells by the translocated CagA protein of H. pylori , and may confer a beneficial effect on the reduction of cancer risk.
- Subjects
BACTERIAL proteins; HELICOBACTER pylori; EPITHELIAL cells; GASTRIC mucosa
- Publication
Alimentary Pharmacology & Therapeutics, 2003, Vol 18, p39
- ISSN
0269-2813
- Publication type
Article
- DOI
10.1046/j.1365-2036.18.s1.2.x