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- Title
Optimization of Extraction Process of Quercetin from Flos Sophorae Immaturus Using Orthogonal Design.
- Authors
Yanyan ZHENG; Ruonan HE; Zhongwei CHEN; Chao YI; Wenwen LU; Zhuangyu ZHANG
- Abstract
[Objectives] This study aimed to optimize the ultrasonic-assisted extraction process of quercetin from Flos Sophorae Immaturus. [Methods] The natural product quercetin in Flos Sophorae Immaturus was extracted by ultrasonic-assisted method, and the content of quercetin in the extract was determined by ultraviolet spectrophotometry. The effect of ethanol concentration, solid/liquid ratio, temperature, time and ultrasonic power on the yield of quercetin in Flos Sophorae Immaturus was investigated by using single-factor experiments. Based on the results of single-factor experiments, an orthogonal experiment was designed to optimize the extraction process. The experimental data obtained were subjected to range analysis, analysis of variance and SSR test using SPSS 20.0 software to obtain the optimal extraction process. The results concluded were verified. [Results] The optimal ultrasonic-assisted extraction process for quercetin in Flos Sophorae Immaturus was as follows: temperature of 85 °C, time of 30 min, solid/liquid ratio of 1:20 g/mL, ethanol concentration of 50 %. Under the optimal extraction conditions, the yield of quercetin in Flos Sophorae Immaturus was the highest. [Conclusions] This study provides a theoretical basis for the application of quercetin in Flos Sophorae Immaturus.
- Subjects
QUERCETIN; ULTRAVIOLET spectrophotometry; PROCESS optimization; NATURAL products; ANALYSIS of variance
- Publication
Medicinal Plant, 2020, Vol 11, Issue 6, p67
- ISSN
2152-3924
- Publication type
Article
- DOI
10.19600/j.cnki.issn2152-3924.2020.06.015