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- Title
Apoptosis of Hepatoma Cells HepG-2 Induced by Shikimic Acid from FRUCTUS ANISI STELLATI and Its Mechanism.
- Authors
ZHU, Kaimei; GU, Shengjiu; LI, Meibo; QI, Junbin; XU, Yourui
- Abstract
[Objective] To research the apoptosis of hepatoma cells HepG-2 induced by shikimic acid from FRUCTUS ANISI STELLATI and its mechanism. [Methods] Hepatoma cells HepG-2 at logarithmic phase were randomly divided into drug group and control group. Shikimic acids from FRUCTUS ANISI STELLATI at the concentrations of 0. 125, 0. 5 and 1 g/L were used. Inhibitory effects of shikimic acids on HepG-2 cell proliferation were detected by MTT method. Effects of shikimic acids at the concentrations of 0. 125, 0. 5 and 1 g/L on the cell cycle of hepatoma cells HepG were researched by Flow Cytometer. Expression of Bcl-2 and Bax proteins were detected by immunocytochemistry detection. [Results] Results of MTT test showed that shikimic acid (0. 125, 0. 5 and 1 g/L) had significant inhibitory effects on the proliferation of in vitro human liver cancer HepG-2 in a time- and dose-dependent relationship (P<0.05). Shikimic acid blocked the hepatoma cells HepG-2 at G1 phase, so that the cells could not enter into S phase for DNA synthesis, which restricted the cell proliferation. Results of immunocytochemistry detection showed that protein expression of Bcl-2 decreased significantly, while Bax expression enhanced significantly, so that the proportion of Bcl-2 to Bax reduced. [Conclusions] shikimic acid blocked the hepatoma cells HepG-2 at Gx phase, and had significant inhibitory effects on the proliferation of human hepatoma cell through reducing the proportion of Bcl-2 to Bax.
- Subjects
APOPTIN; SHIKIMIC acid; CELL proliferation; DNA synthesis; REJUVENESCENCE (Botany)
- Publication
Medicinal Plant, 2014, Vol 5, Issue 6, p16
- ISSN
2152-3924
- Publication type
Article