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- Title
Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation.
- Authors
Erol, Kevser; Yiğitaslan, Semra; Ünel, Çiğdem; Kaygısız, Bilgin; Yıldırım, Engin
- Abstract
Background: Calcium homeostasis is considered to be important in antineoplastic as well as in neurotoxic adverse effects of cisplatin. Aims: This study aimed to investigate the role of Ca2+ in cisplatin neurotoxicity in cultured rat dorsal root ganglia (DRG) cells. Study Design: Cell culture study. Methods: DRG cells prepared from 1-day old Sprague-Dawley rats were used to determine the role of Ca2+ in the cisplatin (10-600 µM) neurotoxicity. The cells were incubated with cisplatin plus nimodipine (1-3 µM), dizocilpine (MK-801) (1-3 µM) or thapsigargin (100-300 nM). Toxicity of cisplatinon DRG cells was determined by the MTT assay. Results: The neurotoxicity of cisplatin was significant when used in high concentrations (100-600 µM). Nimodipine (1 µM) but not MK-801 or thapsigargin prevented the neurotoxic effects of 200 µM of cisplatin. Conclusion: Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.
- Publication
Balkan Medical Journal, 2016, Vol 33, Issue 2, p144
- ISSN
2146-3123
- Publication type
Article
- DOI
10.5152/balkanmedj.2016.161110