We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cryopreservation‐induced morphological changes in the sperm of South American silver catfish (Rhamdia quelen).
- Authors
da Costa, Bruna B.; Marques, Lis S.; Lassen, Paula G.; Rodrigues, Rômulo B.; Streit, Danilo P.
- Abstract
The cryopreservation protocols are species‐specific owing to variable sperm sensitivity towards temperature reduction and contact with cryoprotectant solutions. However, little is known about spermatic pathologies, especially after the cryopreservation process. Thus, the objective was to evaluate the effect of cryopreservation on morphological changes in semen of jundiá (Rhamdia quelen). Sperm pool of five males, with >80% motility, as collected, diluted in a cryoprotectant solution and frozen in liquid nitrogen (−196°C). There was a reduction in the percentage of normal cells and sperm motility, accompanied by an increase in the percentage of sperm abnormalities after cryopreservation of R. quelen spermatozoa, indicating a substantial fragility of the spermatozoa towards the cryopreservation process. The most frequent types of morphological changes in the cryopreserved semen were macrocephaly, folded tail, strongly curled tail and distally curled tail. This is the first study to evaluate the spermatic morphology of R. quelen before and after cryopreservation, paving way for further investigations on morphological alterations and for a new classification of these changes in fish semen due to cryopreservation.
- Subjects
HARDHEAD catfish; FROZEN semen; SPERMATOZOA; CLASSIFICATION of fish; CELL motility; CRYOPRESERVATION of organs, tissues, etc.
- Publication
Journal of Applied Ichthyology, 2019, Vol 35, Issue 4, p987
- ISSN
0175-8659
- Publication type
Article
- DOI
10.1111/jai.13928