We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Construction of a High Titer Infectious HIV-1 Subtype C Proviral Clone from South Africa.
- Authors
Jacobs, Graeme B.; Bock, Stefanie; Schuch, Anita; Moschall, Rebecca; Schrom, Eva-Maria; Zahn, Juliane; Reuter, Christian; Preiser, Wolfgang; Rethwilm, Axel; Engelbrecht, Susan; Kerkau, Thomas; Bodem, Jochen
- Abstract
The Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. We identify the env-region for being the determinant for the higher viral titers and the pZAC Env to be M-tropic. This higher replication capacity does not lead to a higher cytotoxicity compared to previously described subtype C viruses. In addition, the pZAC Vpu is also shown to be able to down-regulate CD4, but fails to fully counteract CD317.
- Subjects
SOUTH Africa; HTLV; HIV; MOBILE genetic elements; CYTOPLASMIC inheritance
- Publication
Viruses (1999-4915), 2012, Vol 4, Issue 9, p1830
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v4091830