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- Title
Identification and characterization of stationary phase- inducible genes in Escherichia coli.
- Authors
Weichart, Dieter; Lange, Roland; Henneberg, Nicola; Hengge-Aronis, Regine
- Abstract
During transition into stationary phase a large set of proteins is induced in <em>Escherichia coli</em>. Only a minority of the corresponding genes has been identified so far. Using the λ<em>plac</em>Mu system and a plate screen for carbon starvation-induced fusion activity, a series of chromosomal <em>lacZ</em>fusions (<em>csi::lacZ</em>) was isolated. In complex medium these fusions were induced either during late exponential phase or during entry into stationary phase, <em>csi::lacZ</em> expression in minimal media in response to starvation for carbon, nitrogen and phosphate sources and the roles of global regulators such as the alternative sigma factor σs (encoded by <em>rpoS</em>), cAMP/CRP and the <em>relA</em> gene product were investigated. The results show that almost every fusion exhibits its own characteristic pattern of expression, suggesting a complex control of stationary phase-inducible genes that involves various combinations of regulatory mechanisms for different genes. All fusions were mapped to the <em>E. coli</em> chromosome. Using fine mapping by Southern hybridization, cloning, sequencing and/or phenotypic analysis, <em>csi-5, csi-17</em>, and <em>csi-18</em> could be localized in <em>osmY</em> (encoding a periplasmic protein), <em>glpD</em> (aerobic glycerol-3-phosphate dehydrogenase) and <em>glgA</em> (glycogen synthase), respectively. The other fusions seem to specify novel genes now designated <em>csiA</em> through to <em>csiF</em>. <em>csi-17(glpD)::lacZ</em> was shown to produce its own glucose-starvation induction, thus illustrating the intricacies of gene-fusion technology when applied to the study of gene regulation.
- Subjects
PROTEINS; CHROMOSOMES; CARBON; NITROGEN; PHOSPHATES; GLUCOSE
- Publication
Molecular Microbiology, 1993, Vol 10, Issue 2, p407
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1993.tb02672.x