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- Title
Transepithelial transport of milk‐derived angiotensin I‐converting enzyme inhibitory peptide with the RLSFNP sequence.
- Authors
Zhu, Qian; Guo, Yuxing; Pan, Daodong; Gan, Junai; Zeng, Xiaoqun; Sun, Yangying; Wu, Zhen
- Abstract
Abstract: BACKGROUND: To exert an antihypertensive effect after oral administration, angiotensin I‐converting enzyme (ACE)‐inhibitory peptides must remain active after intestinal transport. The purpose of this article is to elucidate the transport permeability and route of ACE‐inhibitory peptide Arg‐Leu‐Ser‐Phe‐Asn‐Pro (RLSFNP) across the intestinal epithelium using Caco‐2 cell monolayers. RESULTS: Intact RLSFNP and RLSFNP breakdown fragments F, FNP, SFNP and RLSF were found in RLSFNP transport solution across Caco‐2 cell monolayers using ultra‐performance liquid chromatography–tandem mass spectrometry. RLSFNP fragments FNP, SFNP and RLSF also contributed to ACE inhibitory effects. Protease inhibitors (bacitracin and leupeptin) and absorption enhancers (sodium glycocholate hydrate, sodium deoxycholate and Na2EDTA) improved the transport flux of RLSFNP. A transport inhibitor experiment showed that intact RLSFNP may be transported via the paracellular route. CONCLUSION: Intact RLSFNP can be transported across the Caco‐2 cell monolayers via the paracellular route. Extensive hydrolysis was the chief reason for the low permeability of RLSFNP. © 2017 Society of Chemical Industry
- Subjects
ANGIOTENSIN I; ANGIOTENSIN converting enzyme; PEPTIDES; EPITHELIUM; MONOMOLECULAR films
- Publication
Journal of the Science of Food & Agriculture, 2018, Vol 98, Issue 3, p976
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.8545