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- Title
Synthesis of Non-linear Protein Dimers through a Genetically Encoded Thiol-ene Reaction.
- Authors
Torres-Kolbus, Jessica; Chou, Chungjung; Liu, Jihe; Deiters, Alexander
- Abstract
Site-specific incorporation of bioorthogonal unnatural amino acids into proteins provides a useful tool for the installation of specific functionalities that will allow for the labeling of proteins with virtually any probe. We demonstrate the genetic encoding of a set of alkene lysines using the orthogonal PylRS/PylTCUA pair in Escherichia coli. The installed double bond functionality was then applied in a photoinitiated thiol-ene reaction of the protein with a fluorescent thiol-bearing probe, as well as a cysteine residue of a second protein, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins.
- Subjects
DIMERIZATION; GENETIC code; PROTEIN synthesis; THIOL derivatives; AMINO acids; RECOMBINANT proteins; ESCHERICHIA coli
- Publication
PLoS ONE, 2014, Vol 9, Issue 9, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0105467