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- Title
DDX5 Facilitates HIV-1 Replication as a Cellular Co-Factor of Rev
- Authors
Zhou, Xiuxia; Luo, Juan; Mills, Lisa; Wu, Shuangxin; Pan, Ting; Geng, Guannan; Zhang, Jim; Luo, Haihua; Liu, Chao; Zhang, Hui
- Abstract
HIV-1 Rev plays an important role in the late phase of HIV-1 replication, which facilitates export of unspliced viral mRNAs from the nucleus to cytoplasm in infected cells. Recent studies have shown that DDX1 and DDX3 are co-factors of Rev for the export of HIV-1 transcripts. In this report, we have demonstrated that DDX5 (p68), which is a multifunctional DEAD-box RNA helicase, functions as a new cellular co-factor of HIV-1 Rev. We found that DDX5 affects Rev function through the Rev-RRE axis and subsequently enhances HIV-1 replication. Confocal microscopy and co-immunoprecipitation analysis indicated that DDX5 binds to Rev and this interaction is largely dependent on RNA. If the DEAD-box motif of DDX5 is mutated, DDX5 loses almost all of its ability to bind to Rev, indicating that the DEAD-box motif of DDX5 is required for the interaction between DDX5 and Rev. Our data indicate that interference of DDX5-Rev interaction could reduce HIV-1 replication and potentially provide a new molecular target for anti-HIV-1 therapeutics.
- Subjects
RNA helicase genetics; HIV; COFACTORS (Biochemistry); VIRAL proteins; GENE expression in viruses; RNA splicing; CYTOPLASM; CONFOCAL microscopy; VIRAL replication
- Publication
PLoS ONE, 2013, Vol 8, Issue 5, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0065040