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- Title
Activin B Promotes Epithelial Wound Healing In Vivo through RhoA-JNK Signaling Pathway.
- Authors
Min Zhang; Nu-Yun Liu; Xue-Er Wang; Ying-Hua Chen; Qing-Lin Li; Kang-Rong Lu; Li Sun; Qin Jia; Lu Zhang; Lin Zhang
- Abstract
Background: Activin B has been reported to promote the proliferation and migration of keratinocytes in vitro via the RhoAJNK signaling pathway, whereas its in vivo role and mechanism in wound healing process has not yet been elucidated. Principal Findings: In this study, we explored the potential mechanism by which activin B induces epithelial wound healing in mice. Recombinant lentiviral plasmids, with RhoA (N19) and RhoA (L63) were used to infect wounded KM mice. The wound healing process was monitored after different treatments. Activin B-induced cell proliferation on the wounded skin was visualized by electron microscopy and analyzed by 59-bromodeoxyuridine (BrdU) incorporation assay. Protein expression of p-JNK or p-cJun was determined by immunohistochemical staining and immunoblotting analysis. Activin B efficiently stimulated the proliferation of keratinocytes and hair follicle cells at the wound area and promoted wound closure. RhoA positively regulated activin B-induced wound healing by up-regulating the expression of p-JNK and p-cJun. Moreover, suppression of RhoA activation delayed activin B-induced wound healing, while JNK inhibition recapitulated phenotypes of RhoA inhibition on wound healing. Conclusion: These results demonstrate that activin B promotes epithelial wound closure in vivo through the RhoA-Rock- JNK-cJun signaling pathway, providing novel insight into the essential role of activin B in the therapy of wound repair.
- Subjects
ACTIVIN; PROMOTERS (Genetics); EPITHELIAL cells; WOUND Healing Dependent on Rate of Histamine Formation (Book); JNK mitogen-activated protein kinases; CELLULAR signal transduction; CELL proliferation; KERATINOCYTES; LABORATORY mice
- Publication
PLoS ONE, 2011, Vol 6, Issue 9, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0025143