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- Title
Two Triacylglycerol Pathway Genes, CTDNEP1 and LPIN1, are Down-Regulated by hsa-miR-122-5p in Hepatocytes.
- Authors
Naderi, Mahmood; Pazouki, Abdolreza; Arefian, Ehsan; Hashemi, Seyed Mahmoud; Jamshidi-Adegani, Fatemeh; Gholamalamdari, Omid; Soudi, Sara; Azadmanesh, Kayhan; Samiee, Siamak Mirab; Merat, Shahin; Fesharaki, Mohammad Gholami; Mondanizadeh, Mahdieh; Vasei, Mohammad; Soleimani, Masoud
- Abstract
Background: Expression of miR-122 is highly specific to hepatocytes of the liver. This miRNA is involved in lipid hemostasis of the tissue; however, there is no comprehensive understanding of its function in lipid hemostasis. Materials and Methods: Since hepatocytes are responsible for part of Triacylglycerol (TAG) synthesis in the body, we hypothesized that miR-122, as the most abundant miRNA in the tissue, might regulate TAG metabolism by targeting key enzymes that are involved in its production pathway. A systematic computational analysis of putative targets of miR-122 identified CTDNEP1 and LPIN1 genes in the TAG pathway. V\fe used dual-luciferase reporter assay, quantitative RT-PCR as well as western blot to confirm the repressive effect of miR-122 on CTDNEP1 and LPIN1 in TAG pathway. Results: Real time PCR on liver needle biopsies with hepatosteatosis showed that miR-122 is up-regulated in hepatosteatosis. Surprisingly, the protein and RNA level of identified targets of miR-122 are also up-regulated in clinical samples, probably as a disproportionate feedback response to the high level of miR-122. Conclusion: Our findings suggest that up-regulation of miR-122 can trigger the compensatory response of LPIN1 and CTDNEP1 in hepatosteatosis.
- Subjects
BIOLOGICAL assay; CELLULAR signal transduction; GENES; GENETIC disorders; IMMUNOHISTOCHEMISTRY; LIPIDS; LIPID metabolism disorders; LIVER; NEEDLE biopsy; POLYMERASE chain reaction; T-test (Statistics); TRIGLYCERIDES; WESTERN immunoblotting; DATA analysis software
- Publication
Archives of Iranian Medicine (AIM), 2017, Vol 20, Issue 3, p165
- ISSN
1029-2977
- Publication type
Article