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- Title
Exosomes derived from human dermal fibroblasts protect against UVB-induced skin photoaging.
- Authors
Park, A Yeon; Lee, Jung Ok; Jang, Youna; Kim, Yu-Jin; Lee, Jung Min; Kim, Su-Young; Kim, Beom Joon; Yoo, Kwang Ho
- Abstract
Exosomes are used as innovative treatment options for repairing skin defects, such as aging, atopic dermatitis and wounds. However, the effects of exosomes obtained from human foreskin fibroblasts BJ-5ta (BJ-5ta Exo) on ultraviolet B (UVB)-mediated photoaging have not been previously reported, at least to the best of our knowledge. Therefore, the present study aimed to investigate the anti-photoaging effects of BJ-5ta Exo on UVB radiation in human skin fibroblasts and SKH-1 hairless mice. The results revealed that BJ-5ta Exo decreased the production of reactive oxygen species and inhibited the decrease in the expression levels of superoxide dismutase 1 and 2, glutathione peroxidase and catalase following UVB exposure. In addition, BJ-5ta Exo attenuated the decrease in nuclear factor erythroid 2-related factor 2 levels induced by UVB rays, indicating its scavenging activity against oxidative stress. Moreover, BJ-5ta Exo inhibited the UVB-induced increase in the levels of γH2AX, p53/21 and cleaved PARP, whereas it promoted DNA double-strand break repair through radiation sensitive 52 and effectively activated the TGF-β1/Smad pathway. BJ-5ta Exo also protected against UVB-induced senescence, as indicated by the downregulation in the levels of senescence-associated β-galactosidase and p16. In a mouse model of photoaging, BJ-5ta Exo prevented the UVB-induced increase in transepidermal water loss, wrinkle formation and MMP-1 expression, while also suppressing the UVB-mediated decrease in collagen type I and elastin levels in the dorsal skin. Overall, the findings of the present study suggest that BJ-5ta Exo represent an effective anti-photoaging agent, which can be used as a component in cosmetic products.
- Publication
International Journal of Molecular Medicine, 2023, Vol 52, Issue 6, pN.PAG
- ISSN
1107-3756
- Publication type
Article
- DOI
10.3892/ijmm.2023.5323