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- Title
MORPHOLOGICAL AND MOLECULAR CHARACTERISATION OF XIPHINEMA INDEX THORNE AND ALLEN, 1950 (NEMATODA: LONGIDORIDAE) ISOLATES FROM CHILE.
- Authors
Meza, Pablo; Aballay, Erwin; Hinrichsen, Patricio
- Abstract
Xiphinema index is a major plant parasitic nematode for vineyards, both as a root pathogen and as a vector for Grape Fanleaf virus. In Chile it has a wide distribution, causing considerable damage to Vitis vinifera. The main morphological and morphometric features that have been used for its identification are related to the vulval position and the presence of a mucro on the tail. However, these features have a limited discrimination capacity, as identification is more complicated in a highly complex matrix such as the soil, where a mixture of species coexists. This situation has motivated the search for and the application of molecular techniques with increased resolution power. This investigation shows the results of morphological and molecular characterization of X. index isolates from Chile. The complete ITS regions (ITS1 and ITS2) were sequenced revealing very low intraspecific diversity, less than 1% or the most divergent available sequences. This coincided with the low level of differences detected at the morphometric level.
- Subjects
CHILE; DAGGER nematodes; MORPHOMETRICS; MORPHOLOGY; MOLECULAR structure; PLANT nematodes; GRAPEVINE fanleaf virus
- Publication
Nematropica, 2012, Vol 42, Issue 1, p41
- ISSN
0099-5444
- Publication type
Article