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- Title
Effect of salicylic acid on expression level of genes related with isoprenoid pathway in centella ( Centella asiatica (L.) Urban) cells.
- Authors
Loc, Nguyen; Giang, Nguyen; Huy, Nguyen
- Abstract
In this study, we report the expression level of CaSQS, CabAS and CaCYS, the genes involved in phytosterol and triterpene metabolic pathway of centella ( Centella asiatica (L.) Urban), in cells elicited with salicylic acid (50-200 µM). Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis indicated CaSQS, CabAS, and CaCYS genes expressed in both the wild-type and cultured cells (with and without elicitation). In elicited cells, expressions of CaSQS, CabAS, and CaCYS genes showed strong dependence on salicylic acid concentration and elicitation day. The highest expression of CabAS gene was found in the cells elicited with 100 µM salicylic acid on day 10 of inoculation. Salicylic acid treatment (50-200 µM) decreased expression level of CaCYS and CaSQS genes in elicited cells compared with the control.
- Publication
3 Biotech, 2016, Vol 6, Issue 1, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-016-0404-z